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rat anti mouse il 18rα mab  (R&D Systems)


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    Structured Review

    R&D Systems rat anti mouse il 18rα mab
    Rat Anti Mouse Il 18rα Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+mouse+il+18/Mouse+IL-18+R+alpha%2FIL-1+R5+APC-conjugated+Antibody/pm38587448-29-22-51
    Average 92 stars, based on 4 article reviews
    rat anti mouse il 18rα mab - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Incubation:

    Article Title: NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice.
    Article Snippet: The membranes were blocked using Odyssey® blocking buffer (Odyssey; LI-COR, NE, USA). .. Membranes were incubated overnight at 4 °C, with rat anti-mouse IL-18 (1μg/ml; D047-3; R&D Systems), rabbit anti-human IL-18 (0.2μg/ml; sc-7954; Santa Cruz Biotechnology), mouse anti-human caspase-1 (0.1μg/ml; sc56036; Santa Cruz Biotechnology), rabbit anti-ASC (1μg/ml; clone AL177; AdipoGen, CA, USA), rabbit anti-human NLRP3 (1μg/ml; Clone D2P5E; Cell Signalling, CA, USA) or mouse anti-FLAG (1μg/ml; F1804; Sigma-Aldrich, CastleHill, NSW,Australia).Membraneswerewashed in PBS-0.05% (v/v) Tween 20 and incubated for 2 h with goat-anti-mouse secondary antibody-Alexa Fluor® 680 conjugate or goat anti-rabbit secondary antibody-Alex Fluor® 800 (0.67 ng/ml; ThermoFisher Scientific).Membranes werewashed and developed on theOdyssey Infrared Imaging System (LI-COR). .. As loading controls, lysate samples were probed with rat-anti-human Tubulin (0.9 ng/ml; Rockland, PA, USA) and goat-anti-rat secondary antibody-Alexa Fluor® 800 conjugate (0.33 ng/ml; ThermoFisher Scientific).

    Article Title: NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection
    Article Snippet: .. After 1 hr, sections were incubated overnight at 4oC with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916, Abcam). .. An isotype control for IL-18 staining was performed using mouse IgG (Dako).

    Article Title: NOD1 mediates non-canonical inflammasome processing of interleukin-18 in epithelial cells to Helicobacter pylori infection
    Article Snippet: .. After 1 hr, sections were incubated overnight at 4°C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916, Abcam). .. An isotype control for IL-18 staining was performed using mouse IgG (Dako).

    Article Title: NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice
    Article Snippet: .. After 1 h, sections were incubated overnight at 4 °C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916; Abcam, Boston, MA, USA). ..

    Article Title: NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice.
    Article Snippet: .. After 1 h, sections were incubated overnight at 4 °C with an antibody mix containing rat anti-mouse IL-18 (5μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916; Abcam, Boston, MA, USA). ..

    Article Title: NOD1 mediates non-canonical inflammasome processing of interleukin-18 in epithelial cells to Helicobacter pylori infection
    Article Snippet: The membranes were blocked using Odyssey® blocking buffer (Odyssey; LI-COR, NE, USA). .. Membranes were incubated overnight at 4°C, with rat anti-mouse IL-18 (1 μg/ml; D047-3; R&D Systems), rabbit anti-human IL-18 (0.2 μg/ml; sc-7954; Santa Cruz Biotechnology), mouse anti-human caspase-1 (0.1 μg/ml; sc56036; Santa Cruz Biotechnology), rabbit anti-Asc (1 μg/ml; clone AL177; AdipoGen, CA, USA), rabbit anti-human NLRP3 (1 μg/ml; Clone D2P5E; Cell Signaling, CA, USA) or mouse anti-FLAG (1 μg/ml; F1804; Sigma). .. Membranes were washed in PBS-0.05% (v/v) Tween 20 and incubated for 2 hr with goat-anti-mouse secondary antibody-Alexa Fluor ® 680 conjugate or goat anti-rabbit secondary antibody-Alex Fluor ® 800 (0.67 ng/ml; ThermoFisher Scientific).

    Article Title: NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice
    Article Snippet: The membranes were blocked using Odyssey® blocking buffer (Odyssey; LI-COR, NE, USA). .. Membranes were incubated overnight at 4 °C, with rat anti-mouse IL-18 (1 μg/ml; D047-3; R&D Systems), rabbit anti-human IL-18 (0.2 μg/ml; sc-7954; Santa Cruz Biotechnology), mouse anti-human caspase-1 (0.1 μg/ml; sc56036; Santa Cruz Biotechnology), rabbit anti-ASC (1 μg/ml; clone AL177; AdipoGen, CA, USA), rabbit anti-human NLRP3 (1 μg/ml; Clone D2P5E; Cell Signalling, CA, USA) or mouse anti-FLAG (1 μg/ml; F1804; Sigma-Aldrich, Castle Hill, NSW, Australia). .. Membranes were washed in PBS-0.05% (v/v) Tween 20 and incubated for 2 h with goat-anti-mouse secondary antibody-Alexa Fluor® 680 conjugate or goat anti-rabbit secondary antibody-Alex Fluor® 800 (0.67 ng/ml; ThermoFisher Scientific).

    Imaging:

    Article Title: NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice.
    Article Snippet: The membranes were blocked using Odyssey® blocking buffer (Odyssey; LI-COR, NE, USA). .. Membranes were incubated overnight at 4 °C, with rat anti-mouse IL-18 (1μg/ml; D047-3; R&D Systems), rabbit anti-human IL-18 (0.2μg/ml; sc-7954; Santa Cruz Biotechnology), mouse anti-human caspase-1 (0.1μg/ml; sc56036; Santa Cruz Biotechnology), rabbit anti-ASC (1μg/ml; clone AL177; AdipoGen, CA, USA), rabbit anti-human NLRP3 (1μg/ml; Clone D2P5E; Cell Signalling, CA, USA) or mouse anti-FLAG (1μg/ml; F1804; Sigma-Aldrich, CastleHill, NSW,Australia).Membraneswerewashed in PBS-0.05% (v/v) Tween 20 and incubated for 2 h with goat-anti-mouse secondary antibody-Alexa Fluor® 680 conjugate or goat anti-rabbit secondary antibody-Alex Fluor® 800 (0.67 ng/ml; ThermoFisher Scientific).Membranes werewashed and developed on theOdyssey Infrared Imaging System (LI-COR). .. As loading controls, lysate samples were probed with rat-anti-human Tubulin (0.9 ng/ml; Rockland, PA, USA) and goat-anti-rat secondary antibody-Alexa Fluor® 800 conjugate (0.33 ng/ml; ThermoFisher Scientific).

    other:

    Article Title: NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection
    Article Snippet: The separated proteins were transferred onto membranes using the iBlot transfer system (ThermoFisher Scienti c), as per the manufacturer’s instructions.



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